Comparison of ethylene carbonate and formamide as components of the hybridization mixture in FISH

The protocols for in situ hybridization (ISH) techniques can vary considerably; however, they usually include denaturation and steps. Denaturing compounds are used to reduce temperature, which keeps the proper morphology of preparation. Formamide is most commonly reagent lower melting temperature. substitution toxic formamide a non-toxic ethylene carbonate at 20 % 50 concentration mixture helped obtain high quality result with two sequences characteristic rye, JNK, Bilby. results after hybridization, duration 90 min 16 h, were identical when or mixture. In addition, was eliminated from post-hybridization steps specific signals both probes still obtained.